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Sử dụng Tris-HCl Buffer

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1 Sử dụng Tris-HCl Buffer on Fri Oct 02, 2015 2:49 pm

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Tris-HCl is a buffer that can be used to control the pH of many solutions, including buffers used in ELISAs, cell and tissue lysis buffers, and buffers for fluorogenic assays.

Tris-HCl can be prepared using Tris base (molecular weight: 121.14 g/mol), or Tris-HCl (Tris base which is already combined with HCl in a 1:1 molar ratio, so the molecular weight is 157.6 g/mol).  

It is important to note which form you use to make the solution, because the initial pH of the solution you make depends on which form you use.

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2 Re: Sử dụng Tris-HCl Buffer on Fri Oct 02, 2015 2:55 pm

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Nói tóm lại, Tris-HCL chỉ là dung môi dùng để kiểm soát pH mà thôi.

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3 Re: Sử dụng Tris-HCl Buffer on Fri Oct 02, 2015 2:58 pm

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TRIS Chemical Formula: 

Tris or Tris(hydroxymethyl)aminomethane or THAM is an organic compound with the formula (HOCH2)3CNH2. Tris is extensively used in biochemistry and molecular biology.[1] In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids. It contains aprimary amine and thus undergoes the reactions associated with typical amines, e.g. condensations withaldehydes.





[th]Properties[/th]
Chemical formulaC4H11NO3
Molar mass121.14 g·mol−1
AppearanceWhite crystalline powder
Density1.328g/cm3
Melting point>175-176 °C (448-449 K)
Boiling point219 °C (426 °F; 492 K)
Solubility in water~50 g/100 mL (25 °C)
Acidity (pKa)8.07

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