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Pha dung dịch 1M MgCl2 như thế nào?

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1 Pha dung dịch 1M MgCl2 như thế nào? on Thu Oct 01, 2015 7:47 am

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Preparation of 1M Magnesium chloride (MgCl2) solution

Requirements


MgCl2.6H2O (Molecular weight = 203.30)
Double distilled water
Measuring cylinder
Conical flask / Beaker
Magnetic stirrer
EDTA | Phosphate buffered saline | CaCl2 stock solution | MgCl2 stock solution | MgSO4 stock solution | MnCl2 solution |

Preparation
To prepare, 1 L of 1M Magnesium chloride (MgCl2) solution, weigh 203.30 g MgCl2.6H2O (Molecular weight = 203.30).
Dissolve it in 800 ml double distilled water.

Note : Do not dissolve in 1 l water. In most cases, the volume is increased when solute is dissolved in solvent. It is prefered to dissolve the solute in a small volume of solvent (e.g., 80% of the required volume). After the solute is completely dissolved and pH is adjusted, make up the correct volume with the solvent.
Once it dissolve, make up the volume to 1 L with double distilled water.
Autoclave it.

Storage
Solution can be stored at room temperature.
Applications
In polymerase chain reaction (PCR)
To prepare Magnesium chloride solution of different concentation and volume, follow the table.

Conc. / Volume 100 ml 250 ml 500 ml 1000 ml
10 mM 0.20 g 0.50 g 1.01 g 2.03 g
50 mM 1.02 g 2.54 g 5.08 g 10.16 g
100 mM 2.03 g 5.08 g 10.16 g 20.33 g
0.5 M 10.16 g 25.41 g 50.82 g 101.65 g
1 M 20.33 g 50.82 g 101.65 g 203.30 g

lol!

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2 Re: Pha dung dịch 1M MgCl2 như thế nào? on Thu Oct 01, 2015 7:49 am

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Ở trên chỉ có 1 chú ý là : Đừng đổ ngay vào 1 L dung môi mà pha vào 800ml trước, sau đó điền đầy (fill up to) tới 1 L

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